Identification of a lactoferrin-binding protein in Prevotella nigrescens
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چکیده
منابع مشابه
Hemoglobin binding activity and hemoglobin-binding protein of Prevotella nigrescens
Prevotella nigrescens, lacking siderophores was found to bind to the hemoproteins. The binding was observed also in the envelope which was prepared by sonication of the cell. The binding occurred in the pH-dependent manner; the binding was observed below neutral pHs of the incubation mixtures but only slightly observed in the neutral and alkaline pHs. Furthermore, hemoglobin bound to the envelo...
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Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigresc...
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To survive and multiply within their hosts, pathogens must possess efficient iron-scavenging mechanisms. In the present study, we investigate the capacity of Prevotella nigrescens and Prevotella intermedia to use various sources of iron for growth and characterize the transferrin-binding activity of P. nigrescens. Iron-saturated human transferrin and lactoferrin, but not ferric chloride and the...
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Previous studies have shown that Gardnerella vaginalis can utilize iron-loaded human lactoferrin as a sole source of iron. In this study, G. vaginalis cells were shown to bind digoxigenin (DIG)-labeled human lactoferrin in a dot blot assay. Using the DIG-labeled human lactoferrin, a 120-kDa human lactoferrin-binding protein was detected by Western blot analysis of G. vaginalis proteins. The lac...
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John Jeongseok Yang, M.D., Tae Yoon Kwon, M.D., Mi Jeong Seo, M.T., You Sun Nam, M.S., Chung Soo Han, M.D., and Hee-Joo Lee, M.D. Department of Medicine, Graduate School, Kyung Hee University; Departments of Laboratory Medicine and Orthopedic Surgery, School of Medicine, Kyung Hee University, Kyung Hee University Hospital, Seoul; Department of Biomedical Science, Graduate School, Kyung Hee Univ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1997.tb10350.x